By Marianna D. Gaça
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2005. Detection of Human Influenza A Viruses by Loop-Mediated Isothermal Amplification. J. Clin. Microbiol. 43, 427-430. , 1992. Rapid detection and identification of avian infectious bronchitis virus. J. Clin. Microbiol. 30, 79-84. Part 2 Treatment Using Alternative Medicines 5 Potential of the Phytomedicine Echinacea in the Treatment of Pulmonary Infections and Bronchitis James Hudson University of British Columbia, Canada 1. Introduction Acute bronchitis is generally attributed to certain respiratory viruses, such as influenza virus A or B, respiratory syncytial virus, coronavirus, rhinoviruses, or others, although various bacteria have often been implicated in some cases, either as causative agents or as secondary agents following the initial virus infection (Gwaltney, 2002; Roxas & Jurenka, 2007; See & Wark, 2008).
Specimen type Blood Kidney Lung Total No. of positive samples 88 62 37 187 No. 5) Table 3. Comparative evaluation of RT-LAMP assay with RT-PCR for 187 clinical samples The result indicated detection limit of IBV RT-LAMP was 10 copies/tube (Fig. 1). In addition, the reaction time of RT-LAMP method is 45 min, which is more rapid than conventional RT-PCR, and the reaction only needs a laboratory water bath. Another useful feature was RT-LAMP products could be directly observed by the addition of dyes.
Identification of avian infectious bronchitis virus by direct automated cycle sequencing of the S-1 gene. Avian Dis. 44 (2), 325-335. , 1993. Differentiation of infectiousbronchitis virus serotypes using polymerase chain-reaction and restrictionfragment- lengthpolymorphism analysis. Avian Dis. 37 (1), 194-202. , 2003. Detection of infectious bronchitis virus by multiplex polymerase chain reaction and sequence analysis. J. Virol. Methods. 109, 31-37. Amonoclonal antibody-based antigen capture enzyme-linked immunosorbent assay for identification of infectious bronchitis virus serotypes.
Approaches to Bronchitis by Marianna D. Gaça