By M. A. Vijayalakshmi
Protecting the fundamental ideas of biotechnology, diverse ideas are assessed and numerous functions integrated to supply the reader with a multidisciplinary viewpoint on the way to gaining knowledge of the various chromatographic methods.
content material: Upstream and downstream steps in biochromatography / Mookambeswaran A. Vijayalakshmi --
Gel filtration / Kjell-Ove Eriksson --
Ion alternate interplay biochromatography / E. Boschetti and P. Girot --
Hydrophobic (interaction) chromatography of proteins / Herbert P. Jennissen --
Conformational behaviour of polypeptides and proteins in reversed section and lipophilic environments / Milton W. Hearn --
Affinity chromatography / Jaroslava Turková --
Dye ligand affinity chromatography / Robert ok. Scopes --
Immobilized man made dyes in affinity chromatography / Nikos E. Labrou and Yannis D. Clonis --
Immobilized histidine ligand affinity chromatography / Oliver Pitiot and Mookambeswaran A. Vijayalakshmi --
Immobilized metal-ion affinity chromatography: from phenomenological hallmarks to structure-based molecular insights / Nadir T. Mrabet and Mookambeswaran A. Vijayalakshmi --
Thiophilic interplay chromatography / Sven Oscarsson and Francisco Batista Viera --
Miscellaneous tools in affinity chromatography: half 1: Boronic acids as selective ligands for affinity chromatography / Xio-Chuan Liu and William H. Scouten --
Miscellaneous tools in affinity chromatography: half 2: Shielded affinity chromatography in packed mattress and improved mattress mode / Igor Yu. Galaev and Bo Mattiasson --
Glycobiology and biochromatography / Henri Debray, Gérard Strecker and Jean Montreuil --
Capillary electrokinetic chromatography / Gargi Choudhary and Csaba Horváth --
Imprinted polymers as tailored desk bound stages for affinity separation / Karsten Haupt, Peter A.G. Cormack and Klaus Mosbach --
Computer-aided simulation of biochromatography / Nicolas Voute --
commercial biochromatography: engineering points / F.A.P. Garcia, D.M.F. Prazeres and J.M.S. Cabral --
Validation points in biochromatography / Charles Lutsch --
Biochromatography and biomedical purposes / C. Legallais, Mookambeswaran A. Vijayalakshmi and Ph. Moriniére.
summary: overlaying the fundamental rules of biotechnology, varied strategies are assessed and a number of functions integrated to supply the reader with a multidisciplinary standpoint as a way to learning the numerous chromatographic equipment
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Extra resources for Biochromatography : theory and practice
Superose® 6 (10×500mm column), (■) Superose 6 prep grade (10×530mm) and (▲) Superose 6 prep grade (16×510mm). Dots represents experimental data and lines represents a calculated relationship. Reproduced with permission from Elsevier (Hagel, 1985). Sample concentration In addition to sample volume the loading of a gel filtration column is restricted by the sample concentration. It has been observed that resolution at high sample concentrations is compromised. Nonuniform flow develops when the viscosity of the sample is too high.
1990). , 1998) have recently been at the basis of new ion exchangers to reach better or more rapid separations. In this review several practical aspects are presented with the aim to summarize for the chromatographer the information required to understand phenomena involved in ion exchange chromatography and to optimize the separation. Matrices and ion exchange groups Ion exchangers are composed of a solid porous structure supporting ionizable chemical groups. A wide variety of groups have been described and are classified into two categories: cationic and anionic.
1969) On the determination of molecular weight of protein subunits on Sephadex G-200 in the presence of detergent. Glutamate dehydrogenase. Can. J. , 47, 401–403. O. (1962) Exclusion chromatography. Arch. Biochem. Biophys. , 1, 157–168. M. J. (1994) Viscous fingering in chromatography visualized via magnetic resonance imaging. Chemical Engineering Sciences, 49 (14), 2229–2241. Porath, J. and Flodin, P. (1959) Gel filtration: a method for desalting and group separation. Nature, 183, 1657–1659. Regnier, F.
Biochromatography : theory and practice by M. A. Vijayalakshmi