By Shin J. Oh
As a certified operating within the frontlines of tissue analysis and in daily perform, you wish a reference that offers you useful details in an easy-to-use layout. Containing over three hundred photos, micrographs, and line drawings, together with over 60 colour illustrations, colour Atlas of Nerve Biopsy Pathology offers a transparent photograph of ordinary ideas for nerve biopsies. You get new diagnostic recommendations and clinicopathological correlations all in a framework designed for speedy reference and simple studying. offering all of the functional info you would like from A to Z, colour Atlas of Nerve Biopsy Pathology describes how to:perform a nerve biopsyhandle specimensprocess the nerve via frozen sections, by means of paraffin sections, and through semi-thin sectionsThe e-book delineates the overall pathological beneficial properties of peripheral nerve problems and the diagnostic power and barriers of the nerve biopsy. It information the common pathological positive factors of every affliction through featuring colour pictures and instance circumstances that offer a quick medical historical past and pertinent electrophysiological and different laboratory facts. The exhaustively illustrated colour structure provide you with quick details in achieving a definitive prognosis. in contrast to different books in this topic, this one comprises frozen part fabrics, case histories, teased nerve illustrations, and newly defined neuropathies. the writer brings twenty-five years of expertise within the lab and educating to the improvement of this atlas. within the lab or within the lecture room, at the entrance traces of tissue prognosis, colour Atlas of Nerve Biopsy Pathology offers you the data you wish in actual fact, concisely, and comfortably.
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Extra info for Color Atlas of Nerve Biopsy Pathology
The nerve specimen is frozen in isopentane cooled to -180°C in liquid nitrogen for 15 seconds. Rapid diagnosis is critical in cases of vasculitis since an immunosuppressive therapy should be instituted as soon as the diagnosis of vasculitis is made. 1). Other advantages of the frozen section are easy detection of myelin-digestion chambers and relative ease of preserving the longitudinal sections in a straight alignment. 1 Treatment of nerve biopsy specimens. 5 Frozen section H&E Modified trichrome PASH Congo-red Cresyl-fast-violet Chapter 3 Final Proof 07/13/2001 7:59 AM Page 26 is critical in recognizing segmental demyelination.
Results appear as follows: Sulphatide — golden brown Normal myelin — purple ALKALINE CONGO-RED STAIN See the Congo-red stain for the paraffin section. Cut at 10 µm. Follow the Congo-red staining procedure for the paraffin section with two differences: begin at step 2 and stain in buffered Congo-red stain for 45 minutes. Sections are examined using crossed Polaroid filters; red-stained amyloid deposits show bright green birefringence. Results are as follows: Nuclei — blue Amyloid — deep pink to red Elastic fiber — pink to red Myelin — blue-purple PROCESSING OF THE NERVE FOR SEMITHIN AND ELECTRON MICROSCOPY SECTIONS Always fix the nerve in 4% glutaraldehyde for at least 24 hours before dehydration and embedding.
Modified trichrome stain. ) CHAPTER 4 Figure 7 Normal sural nerve. Axon (a) is somewhat larger in the frozen section than the paraffin section. Again, the axon is rarely visible in the frozen section; sm means small myelinated fiber and lm means large myelinated fiber. Frozen section. Modified trichrome stain. ) CHAPTER 4 Figure 8 Entire nerve fascicle is filled with red myelinated fibers in normal nerve. Frozen section. Modified trichrome stain. ) CHAPTER 4 Figure 10 Normal sural nerve. Entire nerve fascicle is filled with red large-diameter myelinated fibers in the longitudinal cut.
Color Atlas of Nerve Biopsy Pathology by Shin J. Oh